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The Osmosis Lab








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**\Isolation of Individual Colonies Lab**
























Macroscopic view:
I started my experiment on Friday, June 24,2011 at 3:30 p.m.. Unfortunately, after 48 hours of incubation the only culture which had any growth was the S. epidermidis. As you can see from the pictures above my L. acidophilus specimens did not grow anything. These cultures were created first melting the agar in boiling hot water, and then poured the content into petri dishes. After 48 hours of incubation the agar started decrease in size but not in the amount of microbes. Like I stated before my S. cerevisiae cultures did not have any growth. However my S. epidermidis streak culture did an excellent job. There were colonies created in all four quadrants, but the most growth was in the first quadrant. The first quadrant was where I initially started the streak culture. My stock culture was created by removing a colony from my S. epidermidis streak culture. As a result my specimen is slightly cloudy after incubation.





L. acidophilus was innoculated and cultured in MRS broth. S. epidermidis was stored in nutrient broth. After incubating for 24 hours there was no growth in the S. epidermis culture. I was afraid my specimen was not accurate so I attempted the experiment again. Once again there was not any growth in the tube that contained S. epidermis. However there was a significant amount of growth in the L. acidophilus tube. This picture was taken after 48 hours of incubation. The test tube shows a great example of turbidity. The measured the growth of L. acidophilus is 1.6 cm.